AN AUTOMATED, CLOSED SYSTEM FOR GAS ANALYSIS: ASSAY OF CARBON MONOXIDE IN BLOOD.
Abstract
A closed, automated system for the determination of carbon monoxide is described. The technic was found applicable to the assay of large numbers of blood samples with carbon monoxide content ranging from background levels up to lethal concentrations. The limits of assay for this method are 2 to 10 micromoles. CO per 100 ml. of whole blood, which is equivalent to 0.5 to 2.5 p.p.m. (w/w). Samples containing higher concentrations are appropriately diluted with degassed physiologic saline. Analysis of the optical density per micromole % CO obtained over several months using different bloods with hemoglobin concentrations ranging from 11.7 to 13.0 gm. %, showed a maximum deviation from the overall average of less than 0.005 OD. Whole blood recovery data ranged from 97% to 103% with an average value of 99.5%. The basic technic offers an approach to the quantitative assay of a wide variety of body and environmental gases, both those that are toxic and those that are vitally essential. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1967
- Accession Number
- AD0666422
Entities
People
- Dorothy F. Wease
- Eli S. Espinosa
- Marion J. Stansell
Organizations
- United States Air Force School of Aerospace Medicine