IN VITRO DETERMINATION OF PLASMA INSULIN USING 125I AND LIQUID SCINTILLATION SPECTROMETRY.
Abstract
A procedure for the immunoassay of insulin levels using insulin 125I as a tracer was modified to allow the samples to be measured by means of a liquid scintillation spectrometer. The optimal instrument settings and scintillation fluid system were determined. The absolute efficiency was found to be 21.4%. The components making up the sample were individually tested and found to have no effect on the count rate. The percent change in activity with increasing amounts of added cold insulin (over the range 0 to 500 micro U.) was determined using both the liquid scintillation system and the gamma spectrometer. No significant difference was found between the two methods in terms of accuracy or ease of accomplishing the procedure. The two systems are considered interchangeable for counting insulin 125I samples. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1968
- Accession Number
- AD0666904
Entities
People
- Donald F. Logsdon Jr.
- James F. Green
- Orville L. Langford
Organizations
- United States Air Force School of Aerospace Medicine