CELLULAR RESPONSE TO RADIATION.
Abstract
The radiosensitivity and nuclear volumes were determined for seven species of mammals, eight species of insects, and one species of plant, Araucaria excelsa. The following cells were selected as indicators of radiation sensitivity: (1) Mammals -- columnar epithelial cells of the duodenal intestional mucosa; (2) Insects -- endothelial cells lining the mid gut; (3) Plants -- the non-dividing, interphase nuclei of the tunica and outer corpus cell layer of the terminal shoot meristem. The mammals were whole-body irradiated with a 1 MVp x-ray unit; the insects whole-body irradiated with a 300 KVp x-ray unit; and the plant, totally irradiated with Cobalt 60 gamma rays. The interphase chromosome volume (nuclear volume divided by the diploid number of chromosomes) for each species was determined and plotted against its respective LD50. The mammalian species had a positive slope whereas the insect species had a negative slope. The plant, Araucaria, when plotted with the data obtained by Capella and Conger (June, 1966) also had a negative slope. The positive slope obtained with mammalian species indicated that the larger the interphase nuclear volume, the less sensitive the animal was to ionizing radiation. The inverse of this was true for plants and insects. It was concluded that a relationship between LD50 and interphase nuclear volume does exist and would be valuable as a predictor. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1967
- Accession Number
- AD0668686
Entities
People
- Harvey Cromroy
Organizations
- University of Florida