ANTIBODY FORMATION BY TRANSFERRED PERITONEAL CELLS AND SPLEEN CELLS OF MICE. I. TRANSFER OF CELLS FROM IMMUNIZED NON-IRRADIATED DONORS TO SYNGENEIC RECIPIENTS WITH AND WITHOUT ANTIGEN,
Abstract
Peritoneal cells and spleen cells from LAF 1 mice given 3 intraperitoneal immunizations of sheep red blood cells synthesized hemagglutinins after transfer to x-irradiated syngeneic recipients, either with or without a concomitant injection of antigen. Antibody formation by cells transferred with antigen resembled a secondary antibody response in intact animals. Hemagglutinins appeared rapidly and in high titer. Approximately 50% of the antibodies were resistant to treatment with 2-mercaptoethanol. Antibody formation by cells transferred without further exposure to antigen differed in several respects. Hemagglutinin titers were lower. Throughout the period of observation, only 6-25% of the antibodies formed were mercaptoethanol-resistant. In recipients injected with spleen cells, antibodies appeared rapidly, suggesting that mature antibody-forming cells had been transferred. However, in recipients injected with peritoneal cells from the same donors, antibodies were detected only after a delay of several days, which suggested that mature antibody-forming cells had not been transferred. Hemagglutinins were synthesized as well by a suspension containing all types of peritoneal cells as by a fraction consisting almost entirely of lymphoid cells. Recipients of peritoneal cells from donors which had been given either 1 intraperitoneal immunization or 3 intravenous immunizations had no or only low hemagglutinin titers, in contrast to recipients of cells from donors given 3 intraperitoneal immunizations. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 22, 1968
- Accession Number
- AD0669786
Entities
People
- Lottie Kornfeld
- Walter W. H. Weyzen
Organizations
- Naval Radiological Defense Laboratory