CHEMICAL RADIOPROTECTION OF HEMOPOIETIC COLONY-FORMING CELLS: COMPARATIVE EFFECT OF AET, ANOXIA AND URETHAN,
Abstract
The hemopoietic colony-forming unit (CFU) technique of Till and McCulloch was employed to test the radioprotective effect of AET, anoxia and urethan on marrow cells irradiated in vivo. For AET and anoxia, a dose-reduction factor of 1.9 to 2.1 was found. Since the marrow cells were assayed for CFU content immediately after irradiation of the donor, the observed effect can be interpreted as a 'true' radiation dose reduction. By contrast, urethan injection did not increase the survival of marrow CFU assayed immediately after whole-body X irradiation. However, urethan as well as AET, afforded radioprotection of endogenous CFU content of spleen and bone marrow, but not of endogeneous spleen colony count. It is concluded that the mechanism of radioprotection by urethan is fundamentally different from that of AET or anoxia. The data also suggest that mouse bone marrow CFU content, assayed immediately after irradiation, does not necessarily correlate with animal survival. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 04, 1968
- Accession Number
- AD0669787
Entities
People
- Leonard J. Cole
- W. E. Davis Jr.
Organizations
- Naval Radiological Defense Laboratory