AN INSULIN-INHIBITORY FACTOR OF HUMAN BLOOD.

Abstract

An insulin inhibitor has been found in human blood through use of a two-antibody radioimmunoassay. This report presents additional data and compares the inhibitor to other reported inhibitors. Treatment with heat, strong base, or disodium ethylenediamine tetraacetate (EDTA) does not remove inhibition; therefore, the factor is probably not complement C'. At present, it cannot be determined whether the inhibitor acts between insulin and the first antibody, or between the first and the second antibody. Dilution of the sample 1:10 completely removes the inhibition. Recovery curves over a range of 50 to 500 micro units of insulin show 100% recovery at all points. (Author)

Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1968
Accession Number
AD0671521

Entities

People

  • Donald F. Logsdon
  • James F. Green
  • Orville L. Langford

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Chemical Compounds
  • Dilution
  • Ethylenediamine
  • Inhibition
  • Inhibitors
  • Radioimmunoassay
  • Recovery

Fields of Study

  • Biology

Readers

  • Cardiovascular Physiology
  • Molecular and Cellular Biochemistry
  • Organic Chemistry