THE USE OF DARK-FIELD ILLUMINATION IN THE ELECTRON MICROSCOPE FOR MICROORGANISM STRUCTURE STUDY,
Abstract
With the assistance of the accessories that were installed in the electron microscope EM-3 it is possible to receive a dark-field illumination of a given area of an object and to change it to a bright field of the same area in short period of time. The dark-field method in electron microscopy is perspective for investigation of the internal structures of bacterial cells. In the dark field the difference in the character of the electron diffusion by the bodies of the gram-positive and gram-negative bacteria is clearly shown. It is expressed in that the gram-positive and gram-negative forms appear dark in the bright field, whereas in the dark field the gram-positive forms are dark and the gram-negative bright. A combination of the methods of investigating an object in the dark and bright field gives a more complete representation of the object's structure than either of them can give separately. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1968
- Accession Number
- AD0672105
Entities
People
- G. P. Petrova
- K. A. Ponomarev
Organizations
- United States Army Biological Warfare Laboratories