FLUOROMETRIC DETECTION OF BIOLOGIC CHANGES IN IRRADIATED LABORATORY ANIMALS.
Abstract
The excretion of fluorescent products by rodents and primates after exposure to whole-body sublethal 60Co gamma irradiation was investigated. Gel filtration chromatography and thin-layer chromatography were used to fractionate and quantitate radiation-responsive fluorescent urinary products. A major advancement in quantitating fluorescent urine products was achieved by the fluorescence-scanning of thin-layer chromatograms. Certain radiation dose-response relationships were measurable in two major fluorescent fractions from gel filtration chromatography and thin-layer chromatography of rodent and primate urine extracts within twenty-four hours postirradiation. Species variations between rodents and primates may account for different responses of fluorescent urinary products after irradiation. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1968
- Accession Number
- AD0672819
Entities
People
- James A. Demetriou
- John M. Beattie