THE GROWTH OF JAPANESE AND WEST NILE ENCEPHALITIS VIRUSES IN TISSUE CULTURES,
Abstract
The cytopathogenic effect, which was registered from the first passage, was regularly detected in the cultivation of three strains of the JE virus (Ya-47, R-1, K-2) and one strain of the West Nile encephalitis virus in cultures of monkey-heart cells. Practically identical results were noted in a parallel titration of the virus in tissue cultures and in mice. The specificity of the virus's cytopathogenic effect was proved by means of extinguishing it with a homologous immune serum. In the HeLa cells a specific cytopathogenic effect was registered after six cultural passages of the strain R-1. The cytopathogenic effect was retained in subinoculation. The West Nile encephalitis virus induced a cytopathogenic effect in the HeLa cells from the first passage. The Japanese encephalitis virus (strains Ya-47, R-1, K-2, K-4, and 'Nakayama') and the West Nile encephalitis virus propagated in the cultures of skin-muscle tissue of the human and chick embryos, but they did not induce a cytopathogenic effect. The mean titer and the viruses in an intracerebral inoculation of mice (lg LD50) was 5.6 and 5.0 respectively. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1968
- Accession Number
- AD0673019
Entities
People
- Tsi-min Fan
Organizations
- United States Army Biological Warfare Laboratories