GENE TRANSFER IN STRAINS OF PASTEURELLA PSEUDOTUBERCULOSIS

Abstract

Pasteurella pseudotuberculosis strain 32IV accepted the F'lac episome from Escherichia coli strain 23.IOS and behaved as a gene donor in crosses with several different auxotrophs of P. pseudotuberculosis. Some selected donor markers were transferred at frequencies of .0001 to .00001 per donor cell while others appeared not to be transferred. Up to 40% of recombinants were Lac+. Selected recombinants showed differing unselected marker frequencies with differing selected markers; those obtained by using double marker selection showed increased unselected marker frequencies. Some alternative explanations for the origin of recombinants (syntrophic growth, mixed clones, multiple recipient reversions) were not supported by experiment.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1968
Accession Number
AD0673240

Entities

People

  • B. C. Morris
  • T. W. Burrows
  • W. D. Lawton

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Bacteria
  • Bacteriophages
  • Biological Sciences
  • Cells
  • Chemistry
  • Deoxyribonucleic Acids
  • Escherichia
  • Escherichia Coli
  • Gammaproteobacteria
  • Genes
  • Genetics
  • Genotypes
  • Growth Factors
  • Phenotypes
  • Plasmids
  • Rodents

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics
  • Molecular and genetic basis of cancer.