MONOAMINERGIC MECHANISMS IN NERVOUS TISSUE.
Abstract
Selective denervation experiments in combination with the specific histochemical methods for catecholamines and acetylcholinesterase have shown that the feline dilator pupillae is supplied by numerous parasympathetic as well as sympathetic fibers. Probably fibers of both types accompany each other in the strands of the autonomic ground plexus. A technique for simultaneous demonstration of adrenergic and non-adrenergic fibers has been devised. The method rests on the finding that methylene blue, under certain conditions, will preferably stain non-adrenergic fibers. A new method for the cellular localization of histamine has been worked out. It is based on the principle that di-orthophthalaldehyde under dry conditions forms a fluerophore with histamine. The specificity of the method is high. The histamine fluerophore has been characterized in microspectrometric analyses. The mechanism of ventricular fibrillation during induced hypothermia has been studied in further detail. In cat, prenylamine effectively protects against fibrillation, whose the incidence seems to be related to the number of intact adrenergic nerves present in relation to the cardiac muscles. Hypothermia causes a distinct release of transmitter from the cardiac adrenergic nerves. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 15, 1968
- Accession Number
- AD0674298
Entities
People
- Bengt Falck
- Erik Dahl
Organizations
- Lund University