CONTAMINATION OF TISSUE CULTURES WITH PLEUROPNEUMONIA-LIKE ORGANISMS

Abstract

Out of the 49 transplantable cell lines investigated, 45 (91%) were contaminated with mycoplasms. The morphology of mycoplasm colonies, isolated from various tissue cultures, proved to be the same. The influence of high and low temperatures did not rid contaminated cultures of mycoplasm. With the help of monomycin it was possible to purify HEp-2, HeLa and SOTs cell cultures of mycoplasms.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1967
Accession Number
AD0675099

Entities

People

  • I. V. Rakovskaya

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Amniotic Fluid
  • Anti-Bacterial Agents
  • Cell Line
  • Cells
  • Contamination
  • Culture Techniques
  • Health Services
  • High Temperature
  • Infection
  • Literature
  • Low Temperature
  • Microorganisms
  • Rhesus Monkeys
  • Russian Language
  • Scientific Research
  • Tissue Culture
  • Translations

Readers

  • Microbial Pathology
  • Neurodegenerative Parkinson's Disease and Rickettsial Disease handbook, including the data level of dopamine, BC, neurons, and PD.
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation