A STUDY OF CL. BOTULINUM STRAINS OF TYPES A, B, C, D, AND E,
Abstract
The strict specificity of the toxins of types A, B, and E was confirmed in the neutralization reaction. Common toxic components were revealed only in type C and D strains. A study of 107 strains of Cl. botulinum from the collection allowed the typing of only 49 strains, of which 25 were related to type A, 19 -- type B, 2 -- type C, 1 -- type D, and 2 -- type E. It was shown that it is expedient to include type A strain No 35 and type B strain No 364 in the list of industrial strains. Following prolonged storage, 58 out of 107 strains lost their toxigenic properties, which could not be restored by numerous passages on nutrient media. Toxins from Cl. botulinum cultures type A and B in the precipitation reaction in gel formed from 1 to 8 precipitation lines, toxins of type C and D -- 1 line each, and the toxins of type E -- 2 precipitation lines with type E serum and 1 line with type D serum. There was no conformity between toxicity and the number of precipitation lines in agar; the filtrates of strains which had lost the toxigenic properties could preserve the ability to form precipitation lines. It was established by the precipitation reaction in gel that Cl. botulinum types A and B have common antigens. Common antigens were also revealed in types C and D. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1967
- Accession Number
- AD0675115
Entities
People
- F. A. Chertkova
- I. L. Pletneva
- N. D. Svishcheva
Organizations
- United States Army Biological Warfare Laboratories