CLOSTRIDIUM BOTULINUM F. REPORT 4. OBTAINING RABBIT ANTIBOTULINUM SERUM
Abstract
During the hyperimmunization of rabbits with botulinum toxoid type F (for 4 cycles) and then with the type F toxin (for 3 cycles), a type F serum was obtained which had a titer of antitoxin, equaling on the average 115 AU. The titer of serum from rabbits in the control group, which received type F toxoid over a period of 7 cycles of hyperimmunization, equaled on the average 51 AU. No noticeable difference was exposed in the mobility of the electrophoretic fractions in the immune and nonimmune rabbit sera. In the process of immunization the content of protein in rabbit sera increased, due primarily to gamma- and beta-fractions. In the neutralization reaction it developed that the antibotulinum serum type F, taken in a large quantity (80 AU), protected mice from 1 Dlm of type E boutlinum toxin. At the same time it was established that the type E and type F botulinum toxins have a mosaic structure - the type F toxin contained an insignificant amount of the E toxin component, and the type E toxin contains a small amount of type F toxin. In the precipitation reaction in agar an antigenic bond was detected between the type F boutlinum toxin and toxins of types A, B and D. In order to ensure the correct identification of the botulism causative agents, isolated from various objects (soil, food products, patients, carcasses, etc.), it is necessary to include type F serum along with the sera of types A, B, C, and E in the complex of diagnostic type specific antibotulinum toxins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1968
- Accession Number
- AD0675170
Entities
People
- I. M. Mikhailova
- I. M. Mikhaylova
Organizations
- United States Army Biological Warfare Laboratories