LUMINESCENCE METHOD FOR THE DETERMINATION OF PROTEIN IN MILK
Abstract
Factors which reduce the accuracy of the luminescence method of determination of protein in milk were analyzed. Based on spectra of fluorescence, spectra of excitation and polarization of fluorescence of proteins in milk, it was shown that nonactive absorption of exciting light and the light of luminescence during excitation in the region of 250-280 nm should not lead to errors. It was established that the majority of errors in analysis may be caused by differences in quantum yield of fluorescence of tryptophan in milk proteins developing as a result of differences in the dimensions or nature of packing of protein in granules. These differences are eliminated by means of dilution of milk in 8 M urea, leading to disaggregation of the protein and a reduction in the quantum yield of fluorescence.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 30, 1962
- Accession Number
- AD0675198
Entities
People
- P. N. Saloshenko
- S. Konev
- T. I. Lyskova
Organizations
- United States Army Biological Warfare Laboratories