FURTHER STUDY OF SELF-ASSEMBLY IN T2 PHAGE TAIL SHEATHS

Abstract

Sedimentation analysis, viscosity determinations, and electron microscopy showed that treatment of T2 phage tail sheaths with KOH solutions in concentrations of 0.018-0.1 M and brief exposure to 1 M KOH results in stretching of the protein helix which constitutes the cylinder of the tail sheath. This stretching is reversible and it is followed by a decrease in the sedimentation coefficient from 110S to 52S. Increasing the alkali concentration to 2 M and longer exposure to 1 M KOH causes the stretched protein helixes to dissociate into subunits with a sedimentation coefficient of about 1.5 - 2.0S. Subsequent dialysis results in self-assembly of the subunits which ends in the formation of polysheaths.

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Document Details

Document Type
Technical Report
Publication Date
Jun 03, 1968
Accession Number
AD0675330

Entities

People

  • B. F. Poglazov
  • T. I. Nikolskaya

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Assembly
  • Coefficients
  • Dialysis
  • Dissociation
  • Electron Microscopes
  • Electrons
  • Films
  • Materials
  • Measurement
  • Microscopes
  • Microscopy
  • Proteins
  • Sedimentation
  • Self Assembly
  • Tubular Structures
  • Viscosity

Readers

  • Electrical Engineering
  • Fluid Dynamics.
  • Molecular Genetics

Technology Areas

  • Microelectronics