DETECTION OF CL. BOTULINUM WITH THE AID OF LUMINESCENT ANTIBODIES. REPORT 1. SPECIFIC LUMINESCENCE OF CL. BOTULINUM OBTAINED DURING THEIR PROCESSING WITH LUMINESCENT IMMUNE SERUM

Abstract

A conjugate of botulinum rabbit antiserum was obtained with the isocyanate fluorescein possessing immunological specificity. In staining the smears of the Cl. botulinum pure cultures by the luminescent antiserum an intensive green luminescence of these microbes was obtained. While staining the preparations of other anaerobic cultures, E. coli, staphylococci, or anthracoids by the same conjugate, the specific luminescence was absent. In this way it is possible to reveal solitary Cl. botulinum cells in the smears prepared from suspensions with the concentration up to 500,000 microbic bodies per 1 ml. The method of staining by luminescent antibodies makes it possible to identify Cl. botulinum in microscopic preparations.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1966
Accession Number
AD0675854

Entities

People

  • T. A. Kalitina

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Antigens
  • Bacteria
  • Biochemistry
  • Biological Staining And Labeling
  • Chemistry
  • Detection
  • Globulins
  • Immune Serums
  • Intensity
  • Isocyanates
  • Luminescence
  • Microbiology
  • Microorganisms
  • Microscopes
  • Proteins

Fields of Study

  • Biology

Readers

  • Immunology
  • Microbial Pathology