STUDIES ON SOME FACTORS INFLUENCING THE RELIABILITY OF BOTULINE AND TETANUS ANATOXIN TITERS ACCORDING TO THE FLOCCULATION REACTION
Abstract
Inasmuch as serums used in the flocculation reaction are obtained by inoculation of animals with antigen that has been accepted for the production of anatoxin, the results of the flocculation reaction, essentially specific, may become distorted on account of the concurrent reaction of group (bacterial) precipitinogens - precipitins. Therapeutic, antitoxic, antibotuline serums can be used as flocculents, if they are purified and concentrated according to the 'diaferm-3' method, and if they are not of agglutinating strain that has been accepted for the production of proper anatoxins. The ratio of EC/Lf to the serum in question should equal 1 even for least purified anatoxins. The titer of botuline and tetanic anatoxins conforming to the flocculation reaction from the same flocculent serum depends upon a degree of the anatoxin's purity, namely on the antigen content per 1 mg of common nitrogen.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1966
- Accession Number
- AD0675875
Entities
People
- A. A. Vorobev
- Z. A. Voronova