ELECTRON MICROSCOPIC LOCALIZATION OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE ACTIVITY.
Abstract
Magnesium-dependent adenosine triphosphatase (ATPase) activity of unfixed, isolated, intact skeletal muscle mitochondria, frozen-thawed mitochondria, and digitonin-disrupted submitochondrial particles was localized ultrastructurally after incubation in a Wachstein-Meisel reaction medium and correlated with biochemical ATPase activity. The reaction precipitate was localized within the matrix of intact and partially disrupted mitochondria and between membranous fragments in the submitochondrial particles. These findings are consistent with an ATPase activity residing within the headpieces of the mitochondrial cristae with deposition of reaction product within the adjacent matrix. The effects of various activators, inhibitors, nucleotides, and disruptive procedures on this localization are described. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1968
- Accession Number
- AD0676104
Entities
People
- Dale H. Heitkamp
- I. William Grossman