SURVIVAL OF COLONY-FORMING UNITS AND SURVIVAL OF IRRADIATED MICE TREATED WITH AET OR ENDOTOXIN
Abstract
When mice are given radiation exposures producing the hematopoietic syndrome, it is assumed that it is the killing of hematopoietic stem cells and the leuko- and thrombocytopenias which ultimately develop that predispose the animals to infection, hemorrhage and death. The colony-forming unit (CFU) has many attributes of a (the) hematopoietic stem cell, and it might be expected that a high correlation should exist between CFU survival and survival of the animal. Some earlier studies have supported this correlation, whereas, others have not. In the present experiments three methods of CFU enumeration (endogenous, exogenous, and donor) have been used to evaluate this correlation in mice 'protected' with AET or bacterial endotoxin. The results show that the different CFU enumeration procedures yield somewhat different results, yet under certain conditions the LD50's for AET- or endotoxin-treated mice may be predicted within 5 - 10% from CFU survival curves. In spite of the good correlation between CFU survival and probability of survival of the mouse, it is proposed that the CFU is not the stem cell which determines the radiation sensitivity of the mouse.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 21, 1968
- Accession Number
- AD0676507
Entities
People
- Earl J. Ainsworth
- Rex M. Larsen
Organizations
- Naval Radiological Defense Laboratory