ACRIDINE ORANGE RESISTANT MUTANT OF BACTERIOPHAGE LAMBDA
Abstract
During incubation of a mixture of phage lambda and acridine orange in a solution of low ionic force at a pH of 7.6, dark inactivation of the phage takes place. A phage mutant was isolated and described; it is resistant to acridine orange both within and outside the cell. Acquisition of resistance to the dye cannot be the result of impermeability of the mutant's protein membrane for dye molecules. No differences were detected between the absorption spectra and melting curves of nucleic acids in the mutant and wild type phage. Both DNAs modify identically the spectrum of acridine orange.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 29, 1967
- Accession Number
- AD0678084
Entities
People
- I. V. Andreeva
- V. K. Ravin
Organizations
- United States Army Biological Warfare Laboratories