RESEARCH PROGRAM FOR ALERTING DETECTION AND IDENTIFICATION OF PATHOGENS.
Abstract
The objective of this program is to study the feasibility of detecting and identifying pathogens by the use of gas chromatography in conjunction with ultrasensitive detectors. It was established that matabolic products produced by less than a single cell of Bacillus cereus, Streptococcus faecalis, and Proteus vulgaris could be detected in growth medium within 2 hours incubation. The use of cometabilizable substrates, halogen substituted acids, added to the medium resulted in an improvement by orders of magnitude in the detectability of several bacteria previously requiring large numbers; it was also observed that this approach resulted in an increased number of products which enabled better characterization of the organisms. Three peaks were found common to in vivo and in vitro studies of mice inoculated with Staphylococcus aureus, and four with Salmonella typhimurium. The examination of the sera of dogs infected with infectious hepatitus, herpes, and distemper viruses showed a specific response to each virus. The use of different tissue cultures, other gas chromatography liquid phases, and formation of reaction product derivatives shows promise of significantly increasing the probability of distinguishing a wide range. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1968
- Accession Number
- AD0678202
Entities
People
- B. M. Mitruka
- J. E. Smith
- J. R. Gould
- M. Alexander
- R. A. Gilbert
Organizations
- General Electric