A RAPID METHOD OF OBTAINING ANTIBODIES LABELLED WITH FLUORESCENT STAINS

Abstract

This report describes a new method for the isolation of immune euglobulins (antibodies) from agglutinating anti-plague and anti-cholera serums and a new method of purifying the labelled antibodies from the fluorochrome excess. With the new method of isolating and purifying antibodies, the time required to prepare luminescent conjugates is shortened from 3-4 weeks to 3-4 days. The suggested method of preparing fluorochrome-labelled antibodies is realizable in any laboratory, since it requires no special equipment (refrigeration centrifuges and rooms). The advantages of a new luminescent stain -- fluorescein isothiocyanate, over fluorescein isocyanate are shown. The immune euglobulins isolated by the new method were classifiable among the gamma- globulins in their electrophoretic composition.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1968
Accession Number
AD0678896

Entities

People

  • E. I. Velner
  • E. V. Chibrikova
  • I. I. Shurkina
  • P. K. Tabakov

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Air Platforms

DTIC Thesaurus Topics

  • Antibodies
  • Biomedical And Dental Materials
  • Centrifuges
  • Dialysis
  • Dilution
  • Dyes
  • Fluorophores
  • Gamma Globulin
  • Globulins
  • Immune Serums
  • Isocyanates
  • Laser Dyes
  • Low Temperature
  • Materials
  • Precipitation
  • Proteins
  • Scientific Research

Fields of Study

  • Biology

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Immunology
  • Systems Analysis and Design