A STUDY OF THE BIOLOGICAL PROPERTIES OF TRYPANOSOMA RHODESIENSE AND TRYPANOSOMA GAMBIENSE

Abstract

Three enzymes, hexokinase, aldolase and phosphohexoisomerase, were partially purified by a combination of (NH4)2SO4 fractionation, and DEAE-Sephadex column chromatography. These enzymes have been biologically and physically characterized. Since these enzymes are critical trypanosome enzymes, neutralizing rabbit antisera prepared against them have been tested for their ability to passively protect mice. No protection was observed. In addition by similar techniques a protective antigen was partially purified from crude extracts of Trypanosoma gambiense. One of the most significant advances made by these studies was the development of a working hypothesis to account for the death and pathology of T. gambiense infected animals. In synopsis, this hypothesis involved the presence of a trypanosome toxin, delayed-type hypersensitivity and auto-immunity.

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Document Details

Document Type
Technical Report
Publication Date
Nov 13, 1968
Accession Number
AD0680920

Entities

People

  • John R. Seed

Organizations

  • Tulane University of Louisiana

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Animals
  • Antibodies
  • Biomedical And Dental Materials
  • Blood
  • Blood Cells
  • Cells
  • Chromatography
  • Column Chromatography
  • Hypersensitivity
  • Immune Serums
  • Immunity
  • Leukocytes
  • Medical Personnel
  • Pathology
  • Permeability
  • Rodents
  • Survival

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and Cellular Biochemistry