STUDIES ON POLYMERIC STRUCTURE OF HEMOGLOBIN AND ITS RELATION TO THE FUNCTION.
Abstract
A simple and high yield procedure for obtaining the gamma F subunit in a physiologically active form was developed. The isolated gamma F subunits were identified by starch gel electrophoresis, peptide mapping and the characteristic ultraviolet absorption spectrum. The number of the reactive SH group was estimated as one per chain. The oxygen equilibrium was characterized by an increased affinity toward oxygen, and absence of heme-heme interaction and the Bohr effect. The oxygen affinity was found to be lower than that of the beta A subunits. All these findings are essentially the same as those for other single subunit hemoglobins. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1968
- Accession Number
- AD0681213
Entities
People
- Yasunori Enoki