A SIMPLIFIED DETERMINATION OF URINARY TESTOSTERONE UTILIZING COLUMN AND GAS-LIQUID CHROMATOGRAPHY.

Abstract

A simplified and reliable assay for urinary testosterone has been established which requires only one purification by silica gel column chromatography prior to quantification by gas chromatography. The procedure avoids most of the enzyme-inhibiting debris in the urine by preliminary precipitation of the steroidal material with ammonium sulfate. Enzyme hydrolysis reaches near-maximum completion in 18 to 24 hours at 37C. with only 10% the normal dosage of enzyme. (Author)

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1968
Accession Number
AD0681503

Entities

People

  • J. Throck Watson
  • Michael Lofrano Jr

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

DTIC Thesaurus Topics

  • Analytical Chemistry Techniques
  • Chemical Compounds
  • Chromatography
  • Column Chromatography
  • Gas Chromatography
  • Gels
  • Hydrolysis
  • Liquid Chromatography
  • Materials
  • Precipitation
  • Silica Gels
  • Silicon Dioxide
  • Testosterone

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Prostate Cancer Biology.