IN VIVO AND IN VITRO STUDIES ON BME HEMOGLOBIN.
Abstract
Hemoglobin treated with the sulfhydryl reagent BME had a prlonged plasma survival in rats, primarily due to a marked reduction in hemoglobinuria. In nephrectomized rats, the disappearance of BME hemoglobin and untreated hemoglobin was equally prolonged. In dogs, the plasma survival of BME hemoglobin was also prolonged. After human hemoglobin was treated with BME, its rate of auto-oxidation under physiologic conditions was reduced by about half. BME hemoglobin had an extremely high affinity for oxygen, rendering it useless for the physiologic transport of oxygen. Untreated hemoglobin solutions mixed with plasma had a much less marked increase in oxygen affinity, although its oxygen dissociation curve was also significantly to the left of whole blood. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 07, 1968
- Accession Number
- AD0681898
Entities
People
- H. Franklin Bunn
- William T. Esham
Organizations
- United States Army Medical Research Laboratory