THE MIXED LEUCOCYTE INTERACTION CONSIDERED AS AN IMMUNE RESPONSE,
Abstract
A simple method was adapted to the isolation of leucocytes from the peripheral blood of the mouse and rat. The relatively low ratio of erythrocytes to leucocytes, so obtained afforded optimal responses in the mixed leucocyte interaction. A critical concentration of 2.0 per cent syngeneic mouse plasma in the presence of 2.0 per cent unfiltered foetal bovine serum as culture medium supplement was found to promote optimal responses in the mouse mixed leucocyte interaction. Also, a critical concentration of 1.6 per cent cyngeneic rat plasma applies to this interaction between cells of the rat. Proliferative responses in mouse mixed parental - F1 hybrid cell cultures were found to be approximately half that derived from allogeneic mixed parental - parental cell cultures. The responses of cells from presensitized parental donors cultured with F1 hybrid cells were found to be greater, started earlier and extended longer than those from normal donors. Magnitudes of response of mixed leucocyte interactions of various combinations tested were found consistent with their respective differences in allogeneic specificities. Two per cent foetal bovine serum alone as medium supplement does not support the mouse or rat mixed leucocyte interaction whereas, it supports maximal responses in PHA - cultures of such cells. This differential response of the mixed leucocyte interaction sets the latter apart from the non-specific mitogenic response of peripheral leucocytes to PHA. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 10, 1968
- Accession Number
- AD0682475
Entities
People
- Marjorie J. Jones
- Raymond K. Main
Organizations
- Naval Radiological Defense Laboratory