COATED CHARCOAL RADIOIMMUNOASSAY FOR PLASMA INSULIN,
Abstract
A radioimmunoassay using coated charcoal was developed as a simple, precise, and reproducible technic for measuring plasma insulin. Materials used included insulin-125I, guinea pig anti-insulin serum, and coated charcoal. Varying reagent volumes were tested to achieve an antibody concentration which would bind 60% to 80% of the labeled antigen. The radioimmunoassay consisted of three phases: (1) addition of reagents and a 60-hour incubation period, (2) separation of the antibody-insulin complex from the unbound insulin, and (3) determination of activity and calculation of the insulin concentration. The fasting value found with 10 control subjects ranged from 12 to 72 micro-U./ml. This procedure is adaptable for use as a routine diagnostic test by most radioisotope laboratories. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1968
- Accession Number
- AD0684175
Entities
People
- Donald F. Logsdon Jr.
- Guy M. Strong
- James F. Green
Organizations
- United States Air Force School of Aerospace Medicine