OPTIMIZATION OF A SYNTHETIC NUTRIENT MEDIUM FOR CULTURING TYPHOID BACILLI. REPORT 1: SELECTION OF THE MOST ESSENTIAL FACTORS OF A NUTRIENT MEDIUM BY THE METHOD OF ACCIDENTAL BALANCE,
Abstract
The Satterthwaite random balance method was employed to select an optimum nutrient medium for culturing typhoid bacteria. The addition of five supplementary nutrients (dl-aspargic and dl-glutamic acids, dl-lysin, dl-methionine and l-cistine) to the Troitskiy-Gekker synthetic medium resulted in a six-fold increase in microbial mass. On the basis of the Satterthwaite method it was determined that the five factors most influencing the intensity of propagation were: l-tryptophane, dl-asparagic acid, glucose, bisubstituted ammonium citrate and nicotinic acid. Dl-lysin was omitted from the medium because of its competitive interaction with nicotinic acid. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 18, 1969
- Accession Number
- AD0684245
Entities
People
- B. Yu. Shuster
- V. V. Biryukov
Organizations
- United States Army Foreign Science and Technology Center