GROWTH OF PLANT CELL CULTURES. 3. GROWTH KINETICS AND MASS CULTURE

Abstract

Suspension cultures of bean and lettuce cells have been maintained by serial transfer for over three years. Such cultures may show exponential growth, although growth rates are low with doubling times of three to four days. These suspension cultures have also been grown in commercial laboratory fermenters in batch and semi-continuous systems for period of up to 61 days without contamination. Productivity in fermenters was up to 2.3 grams dry weight of cells per liter of culture per day. Cell yields are up to 40% based in the weight of sucrose in the medium.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1968
Accession Number
AD0684610

Entities

People

  • Hamed M. El-bisi
  • Mary Mandels
  • Robert O. Matthern

Organizations

  • United States Army Soldier Systems Center

Tags

Communities of Interest

  • Biomedical
  • Ground and Sea Platforms

DTIC Thesaurus Topics

  • Accuracy
  • Cell Count
  • Cell Size
  • Chemical Synthesis
  • Chemistry
  • Culture Media
  • Culture Techniques
  • Filters
  • Inoculation
  • Massachusetts
  • Materials
  • New Brunswick
  • Plant Cells
  • Production
  • Productivity
  • Sampling
  • United States

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