NEW STAINING TECHNIQUE FOR SPLEEN NODULES: A COMPARISON OF Do'S,
Abstract
The colony-forming unit (CFU) is thought to be a (the) hematopoietic stem cell, and CFU methodology has been used extensively to evaluate radiation damage to the hematopoietic system in mice. Although the method works satisfactorily, the counting of macroscopic spleen nodules is laborious, time-consuming, and, to a certain extent, subjective. A staining-clearing method has been developed which greatly facilitates and renders this method less tedious and more reproducible. The spleen nodules appear as blue-green spheres in a nearly colorless spleen parenchyma. Estimates of radiation sensitivity (Do) of CFU are not altered by the use of the staining-clearing procedure. However, estimates of the CFU count nucleated marrow cells, or CFU content/femur, are reduced when the staining technique is used. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 28, 1969
- Accession Number
- AD0684771
Entities
People
- Earl J. Ainsworth
- Rex M. Larsen
Organizations
- Naval Radiological Defense Laboratory