BIOPHYSICAL AND BIOCHEMICAL STUDIES ON MICROSOMES OF NERVOUS TISSUE.
Abstract
The light scattering studies were performed on microsomal particles isolated from rabbit brains. It was demonstrated that the measurement of dissymmetry coefficient as well as of angular scattering patterns afforded a means to estimate, at least semiquantitatively, changes in volume of microsomal vesicles. In hypertonic sucrose or salt media, Boyl-van't Hoff's relation was found to be applicable to the osmotic behaviour of brain microsomes. ATP and ADP caused contraction of microsomes in the presence of a monovalent cation. Participation of Na-K-dependent ATPase in such a microsomal contraction could not be proved, though some enzymic process is highly probably involved. The preliminary results strongly suggest that the antimeycin-insensitive electron transport system in brain microsomes described previously is closely related to volume changes of microsomes. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1969
- Accession Number
- AD0684959
Entities
People
- Akira Inouye
Organizations
- Kyoto University