LYMPHOCYTE TRANSFORMATION: A BASIS FOR IN VITRO HISTOCOMPATIBILITY TESTING,
Abstract
One-way stimulation is achieved in mixed leukocyte culture (MLC) tests if cells of one subject are treated with Mitomycin-C and the response is assayed by studying the incorporation of radioactive thymidine. Treated cells cannot incorporate tritiated thymidine but can stimulate untreated allogeneic cells to do so. With this method 29.2% of 184 sibling cell mixtures have not stimulated, whereas all of 226 unrelated cell mixtures have stimulated. Nonstimulators are clearly separated from stimulators. Genetic analysis of these data, based on predicted percentages of nonstimulators (for different genetic relationships) depending on the number of loci and alleles involved, suggests that reactivity in MLC tests is controlled by a single locus with 15 or more alleles. Siblings that do not stimulate (MLC-identical siblings) are also identical for the majority of leukocyte antigens detected by antisera of Amos. The MLC-identical siblings show prolonged skin-graft survival (15 to 36 days) compared with siblings whose cells stimulate in MLC tests (11 to 13 days). The previous studies, combined with the findings of Silvers, Wilson, and Palm, that stimulation in MLC tests in rats only occurs when two rats differ at the major histocompatibility locus, suggests that MLC tests in man measure antigenic differences at the major histocompatibility locus in man. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1968
- Accession Number
- AD0685046
Entities
People
- Fritz H. Bach
Organizations
- University of Wisconsin–Madison