GLYCEROL METABOLISM IN THE PLAGUE MICROBE
Abstract
The GL(+) strain of the plague microbe oxidizes glycerol, glyceraldehyde, and glycerophosphate, but practically does not oxidize dihydroxyacetone. The GL(-) strain does not oxidize these substrates at all. As unique sources of carbon the GL(+) strain uses glycerol, glycerophosphate, and 3-phosphoglyceric acid. The GL(-) strain uses only the last substrate. The capacity to oxidize glycerol is strengthened considerably during incubation of the GL(+) strain on a medium with glycerol. Oxidation of glycerol is accompanied by the formation of pyruvic acid and lactic acid. Oxidation of glycerol is inhibited by bromo- and iodoacetic acid; such an effect is not exhibited by dinitrophenol, fluoroacetamide, and sodium fluoride. The influence of the nature of the buffer on the breakdown of glycerol is manifested only in the fact that in a veronal buffer there is a greater accumulation of pyruvic acid than in the others. In a veronal buffer the greatest rate of oxidation lies within the range of pH 7.0--9.0.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 14, 1969
- Accession Number
- AD0685369
Entities
People
- E. P. Golubinskii
- I. V. Domaradskii
- L. V. Linnikova
Organizations
- United States Army Biological Warfare Laboratories