HOG CHOLERA VIRUS ATTENUATION IN TISSUE CULTURE,
Abstract
Hog cholera virus has been successfully attenuated in tissue culture. After adaptation of the crude virus to cultures of hog embryo kidney cells, it was passaged 30-40 times. Virulence was retained, while the virus became cytopathogenic and only slightly viable. During stabilization by means of 7-fold passaging of cultured virus to a baby pig, the virus established its potential viability. The stabilized virus was repeatedly passaged 5 times in hog embryo kidney cell culture. After secondary stabilization of cultured virus was achieved by means of a single passage through a baby pig, blood from this pig was used as a source of virus for adaptation to transplanted cells. Injection of this virus immunized baby hogs against subsequent infection by crude virus. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 10, 1969
- Accession Number
- AD0685663
Entities
People
- A. A. Pogosyan
- A. B. Khachatryan
- V. G. Airapetyan
Organizations
- United States Army Foreign Science and Technology Center