DETOXICATION OF A PURIFIED AND CONCENTRATED DIPHTHERIA TOXIN BY FORMALIN,

Abstract

Results are presented from a study of the preparation of purified and concentrated diphtheria anatoxin by detoxication of purified toxin. Acid precipitation was used to purify the toxin. Loss of activity in the supernatant fluid and the precipitate did not exceed 8-10%. Depigmented, sterile solutions of purified diphtheria toxin were used for further study of conditions of detoxication, including the role of pH in the conversion of purified toxin to anatoxin. The most favorable results of detoxication were obtained at pH 7.0-7.2. (Author)

Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1969
Accession Number
AD0685814

Entities

People

  • A. A. Vorobev
  • L. V. Larionova
  • N. A. Kalinskaya
  • N. N. Vasilev
  • S. V. Chevotareva

Organizations

  • United States Army Foreign Science and Technology Center

Tags

DTIC Thesaurus Topics

  • Actinomycetales Infections
  • Bacterial Infections
  • Bacterial Infections And Mycoses
  • Chemical Precipitation
  • Chemical Reactions
  • Conversion
  • Precipitates
  • Precipitation

Fields of Study

  • Biology

Readers

  • Immunology
  • Microbial Pathology