ADVANTAGES OF PROPAGATING RABIES VIRUS IN ROLLER SUSPENSION CULTURES IN CELL LINE BHK-21 WHEN USING THE METHODS OF SUBCULTURES AND MIXED CULTURES OF INFECTED AND NON-INFECTED CELLS,
Abstract
A method is proposed for propagating rabies virus in roller-suspension cultures, instead of the standard stationary monolayer techniques. Both the subculture and cell-mixing methods were used with hamster kidney fibroblasts of the VNK-21 line. Primary infection of cells was achieved by addition of a 10% suspension of infected brain, and after nine passages, virus titers up to 10 to the 7.29 power LD50/0.03 ml were achieved. The advantages of the roller-suspension method are: (1) higher virus titers; (2) accumulation of maximum titers 40% faster than in monolayers; (3) easier detection of specific rabies virus antigen by immunofluorescence, appearance of cytoplasmic incorporations and development of a cytopathic effect. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 06, 1969
- Accession Number
- AD0686140
Entities
People
- N. N. Sokolov
Organizations
- United States Army Foreign Science and Technology Center