INSULIN-INHIBITORY FACTOR IN HUMAN BLOOD: ROLE IN THE CHARCOAL RADIOIMMUNOASSAY.

Abstract

The insulin-inhibitory factor in human blood was studied. The results of a recovery study with a single-antibody assay (the charcoal radioimmunoassay) were compared to those with a two-antibody assay to determine whether inhibition occurred between insulin and the first antibody or between the first and the second antibody. The single-antibody assay did not show an insulin-inhibitory factor, but the two-antibody radioimmunoassay did show an inhibitor. The inhibitor must therefore act between the anti-human (gamma globulin) antibody and the anti-insulin antibody to block the reaction. The exact nature of the inhibitor is as yet unknown. (Author)

Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1968
Accession Number
AD0686731

Entities

People

  • Donald F. Logsdon Jr.
  • James F. Green

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

DTIC Thesaurus Topics

  • Amino Acids Peptides And Proteins
  • Antibodies
  • Biomolecules
  • Biopolymers
  • Chemical Compounds
  • Gamma Globulin
  • Globulins
  • Inhibition
  • Inhibitors
  • Macromolecules
  • Molecules
  • Polymers
  • Proteins
  • Radioimmunoassay

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and Cellular Biochemistry