A SIMPLIFIED METHOD OF TRYPSINATION AND GROWING OF RABBIT KIDNEY CELL MONOLAYER CULTURES

Abstract

The authors recommend the hot (37 degrees) single-step method for trypsinization of kidneys of baby and adult rabbits. This method was found to be superior to others tried in yield of cells per gram of tissue, trypsin consumption, growth achieved, and economy. The use of Hottinger muscle digest as the nutritive medium for cultivation insures great availability of rabbit cultures for institutes and practical laboratories. The use of monolayer rabbit kidney cultures in veterinary virology is promising because of the possibility of creating standard conditions for obtaining tissue.

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Document Details

Document Type
Technical Report
Publication Date
Apr 09, 1969
Accession Number
AD0687218

Entities

People

  • I. S. Kuchmasov
  • M. S. Yudina

Organizations

  • United States Army Foreign Science and Technology Center

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Acids
  • Anti-Bacterial Agents
  • Cells
  • Citric Acid
  • Diseases And Disorders
  • Filters
  • Hydrochloric Acid
  • Indicator Dyes
  • Intact Stability
  • Monomolecular Films
  • Mouth Diseases
  • Muscle Proteins
  • Potassium Dichromate
  • Standards
  • Tissue Culture
  • Tissues
  • Virology

Readers

  • Cardiovascular Physiology
  • Microbial Pathology
  • Systems Analysis and Design