TOXIN FORMATION OF Cl. PERFRINGENS TYPES A AND D ON SYNTHETIC MEDIA,
Abstract
A synthetic medium is proposed, which contains no protein or peptones, for the study of growth and toxin formation of Cl. perfringens. On this medium, lecithinase, O-Hemolysin, and e-toxin were obtained in sufficient titres for in vitro and in vivo analysis. Maximum titres were obtained between the 4th and 6th hours of culture growth. Protein was not detected until the 3rd to 4th hour of growth, reached a maximum by the 5th to 6th hour, and decreased after 24 hours. Vitamin B complex promoted accelerated growth with normal morphology in the strains studied, but did not enhance toxin formation. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1969
- Accession Number
- AD0687219
Entities
People
- B. D. Bychenko
- L. G. Ivanova
Organizations
- United States Army Foreign Science and Technology Center