MAXIMUM RATE KINETIC ANALYSIS APPLIED TO ENZYME ASSAY DATA

Abstract

Double reciprocal plots of enzyme concentration vs. reaction velocity provide a rational basis for determining enzyme concentrations by assay methods based on measurement of reaction rates. Maximum rate kinetics furnishes a theoretical basis for the procedure, which is not limited to specific ranges of substrate concentration. Constants somewhat analogous to those of the Michaelis expression for characterizing the enzyme reactions are useful by-products of the method. The hydration of CO2 by bovine carbonic anhydrase illustrates the applicability of the technique.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Feb 05, 1969
Accession Number
AD0688115

Entities

People

  • Donald V. Tappan

Organizations

  • Naval Submarine Medical Research Laboratory

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Biomedical Research
  • Chemistry
  • Classification
  • Clinical Medicine
  • Equations
  • Navy
  • Security
  • Steady State
  • Submarine Bases
  • Submarines
  • Substrates
  • Test And Evaluation

Fields of Study

  • Biology

Readers

  • Cardiovascular Physiology
  • Neurotoxicology
  • Regression Analysis.