SINGLE VS. DOUBLE ANTIBODY RADIOIMMUNOASSAYS FOR PLASMA INSULIN,
Abstract
The double antibody assay and the charcoal assay for plasma insulin were compared as to sensitivity, expense, convenience of use, and adaptability to assay other hormones. The double antibody assay had an overall sensitivity of 0.36%/0.1 micro units, with a sensitivity in the 0 to 1 micro units range of 1.2%/0.1 micro units. The charcoal assay sensitivity was 0.25%/0.1 micro units. The double antibody assay detected down to 0.8 micro units/ml in contrast to 4 micro units/ml. with the charcoal assay. The double assay cost twice as much as the charcoal assay, took more man-hours to complete, and was more difficult to use. The charcoal assay was superior for long-term, high-volume use, and was more easily adaptable to the assay of other hormones. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1969
- Accession Number
- AD0688372
Entities
People
- Donald F. Logsdon Jr.
- James F. Green
- John W. Harper
Organizations
- United States Air Force School of Aerospace Medicine