AN AUTOMATED METHOD WITH A MICROADAPTATION FOR CHOLINESTERASE ASSAYS IN THE DOG.

Abstract

A method for the automated assay of whole blood, plasma, or red blood cell (RBC) cholinesterase in blood samples from the dog is described. The procedure is rapid, sensitive, and highly reproducible. The effects of substrate concentration, hemolysis of RBC's, washing of RBC's, and sampling speeds have been evaluated. Normal cholinesterase levels, and normal variations in canine RBC and plasma cholinesterase during a 2-week period are given. A micromethod has also been developed; it requires 75 microliters of whole blood, plasma, or RBC's for cholinesterase assay. (Author)

Document Details

Document Type
Technical Report
Publication Date
May 01, 1969
Accession Number
AD0688602

Entities

People

  • F. Prescott Ward
  • Thomas L. Hess

Tags

DTIC Thesaurus Topics

  • Blood
  • Blood Cells
  • Cells
  • Hemolysis
  • Sampling
  • Substrates

Fields of Study

  • Medicine

Readers

  • Analytical Chemistry
  • Cardiovascular Physiology