PURIFICATION OF RAT GAMMA GLOBULIN AND THE PRODUCTION OF A SPECIFIC ANTI-RAT GAMMA GLOBULIN SERUM

Abstract

A method for the isolation of rat 7S gamma globulin based on multiple adsorptions with DEAE-Sephadex A-50 has been presented. The separation of rat 7S globulin requires a procedure modified from the one used for humans in terms of the buffer pH, and the number of adsorptions. Ultracentrifugal analysis indicates the resulting fraction is a single component. In addition, immunoelectrophoresis of the fraction when tested against anti-rat sera confirms the homogeneity of this 7S fraction. A monospecific anti-serum to rat 7S gamma globulin has been produced. This antiserum is free of reaction with all other serum components when subjected to immunoelectrophoresis against normal rat serum.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1969
Accession Number
AD0689097

Entities

People

  • E. D. Exum
  • R. T. Bowser

Organizations

  • Armed Forces Radiobiology Research Institute

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Antibodies
  • Biological Sciences
  • Biomedical And Dental Materials
  • Blood Proteins
  • Chemistry
  • Gamma Globulin
  • Health Services
  • Immune Serums
  • Immunoglobulins
  • Materials
  • Military Research
  • Navy
  • Nuclear Medicine
  • Polymeric Films
  • Proteins
  • Public Health

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and Cellular Biochemistry