FORMS OF CLOSED CIRCULAR DNA IN RAT LIVER DURING REGENERATION AND FOLLOWING AMINOAZO-DYE CARCINOGENESIS

Abstract

The closed circular forms of DNA of rat liver have been observed during aminoazo-dye carcinogenesis, during regeneration following partial hepatectomy and in control animals. Paucidisperse multiple mitochondrial forms were not observed. Polydisperse smaller molecules were observed following treatment with chemical carcinogens. The cumulative frequency histogram shows the same profile as others observed in HeLa cells. The relative quantity of the small circles to the mitochondrial circles normally present was extremely small. No conclusions could be drawn regarding the source of these molecules. We conclude that neither regeneration nor carcinogenesis results in an alteration of the genetic recombination apparatus of a magnitude which might yield significant quantities of the two aberrant forms of closed circular DNA.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1969
Accession Number
AD0689451

Entities

People

  • Charles R. Wilson
  • James G. Wetmur

Organizations

  • United States Army Aeromedical Research Lab

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Animals
  • Biomedical Research
  • Cell Division
  • Cell Line
  • Cells
  • Corporations
  • Electron Microscopes
  • Electron Microscopy
  • Electrons
  • Invasive Species (Fauna)
  • Leukemia
  • Materials
  • Microscopes
  • Microscopy
  • Molecules
  • Neoplasms
  • New England

Fields of Study

  • Biology

Readers

  • Aerosol Science/Aerosol Physics
  • Molecular Biology and Genetics
  • Molecular and Cellular Biology

Technology Areas

  • Biotechnology