HOST-PARASITE INTERACTIONS AND GENETICS OF ACTINOPHAGES.
Abstract
The life cycle of phages for streptomycetes and nocardiae were experimentally divided into distinct stages. These included: reversible attachment, irreversible attachment, injection, eclipse, synthesis of viral structural components, maturation, and release. We have confirmed that methylene blue photoinactivates bacterial viruses by photochemical destruction of guanine. In addition, it was shown that riboflavin photochemically destroys guanine. The mechanism of proflavine remains unresolved. In either the light or dark, proflavine binds to adenine or thymine residues of DNA. It was not possible to detect photochemical destruction of a nucleotide or cross-linking of the DNA. The mechanism of action of crystal violet is also not resolved. It binds preferentially to DNA high in guanine and cytosine. No photochemical destruction of a nucleotide or cross-linking of the DNA has been detected. Several lysogenic strains of Streptomyces violaceoruber have been recognized. A mutant sensitive to these temperate phages has been discovered. Some lysozenic strains can be induced by mitomycin C. The temperate phages resemble other actinophages. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 25, 1969
- Accession Number
- AD0690221
Entities
People
- Sterling Gaylen Bradley
Organizations
- University of Minnesota