STUDIES ON THE HYDROLYSIS AND ABSORPTION OF FOLIC ACID POLYGLUTAMATES IN TROPICAL SPRUE.
Abstract
A method was found for the isolation of folic acid polyglutamate from yeast extract. Utilizing this substrate a reproducible assay for 'conjugase' (gamma glutamyl carboxypeptidase) has been established. The pH optima, buffer specificity and gastro intestinal distribution have been shown. Differentiation from a related enzyme, gamma glutamyl transpeptidase has been shown. Discovery that this enzyme (conjugase) is inhibited by dihydoxy bile acids is included, providing a possible explanation for bacterial production of folic acid deficiency. Effective inhibition by BSP of this enzyme has been shown in vitro and vivo. Absorption of folic acid polyglutamate in two patients with tropical sprue was shown to be ineffective and this was corrected by tetracycline but not folic acid. The observation of 'brown serum' in the anemic phase of the disease was studied and explained. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1969
- Accession Number
- AD0690228
Entities
People
- Leslie H. Bernstein
- Sidney Gutstein
- Simeon Pollack
- Simon V. Weiner
Organizations
- Albert Einstein College of Medicine