PROPERTIES OF GENETIC RECOMBINANTS (E. COLI WITH SHIGELLA FLEXNERI) IN THE BODIES OF LABORATORY ANIMALS
Abstract
The purpose of the present work is the isolation of genetic recombinants from the urinary bladder of guinea pigs and the intestines of white mice by means of crossing coliform Hfr strains with Shigella flexneri and studying their biochemical, serological, and pathogenic properties, and also type of nourishment and streptomycin resistance. Of the various crosses 39 genetic recombinants were obtained. All the resulting recombinants were prototrophic and streptomycin resistant. The recombinants fermented lactose, and investigation of the serological properties of the recombinants showed that they were agglutinated by adsorbed Flexneri serum. An investigation of the pathogenic properties of initial strains of Shigella flexneri No 2730 and 569, isolated from recombinants No 1, 2, and 3 from cross Hfr CX2780, and recombinant No 4 from the cross Hfr CX569, showed that they possess pathogenic properties. The results of our investigations testify to the feasibility of interspecies hybridization of bacteria in the living organism. They also make it possible to draw the conclusion that the given mechanism partly explains the formation of pathogenic atypical dysenteric cultures.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1969
- Accession Number
- AD0690256
Entities
People
- A. A. Abidov
- E. F. Kalinina
- M. A. Abdusamatov
- N. A. Zakirov
Organizations
- United States Army Biological Warfare Laboratories