ENHANCEMENT OF VIRAL QUANTITATION BY MEDIA SUPPLEMENTS AND BUFFERS.
Abstract
The incorporation of protamine sulfate (0.8 mg./ml.) in agar overlay medium enhanced the plaque assay of strains of Mengo virus, Sindbis virus, Western equine encephalomyelitis (WEE) virus and vesicular stomatitis (VS) virus. Plaque numbers of Mengo virus and Sindbis virus were increased, while the plaque numbers of WEE virus and VS virus were not altered. The plaque size of Mengo virus and WEE virus was increased, while the size of Sindbis virus plaques was not altered. Plaques of virus were decreased in size to the extent that more plaques could be accommodated per culture. The addition of arginine (0.3 mg./ml.) to agar overlay medium in the absence of protamine did not stimulate Mengo virus plaque formation and actually inhibited plaque formation by WEE virus. Sindbis virus infectivity was stable at 37C. for 2 hours in phosphate buffered saline (PBS) without supplement but not in borate saline buffer (pH 9.0) containing bovine serum albumin (BSA) (up to 400 micrograms/ml.). Mengo virus was stable at 37C. for 2 hours in PBS containing BSA at concentrations of 10, 100, or 400 micrograms/ml., but its infectivity was diminished by incubation in PBS without supplement or in PBS containing sucrose (up to 50 mg./ml.). (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1969
- Accession Number
- AD0690302
Entities
People
- Michael A. Nash
- Patton T. Allen
Organizations
- United States Air Force School of Aerospace Medicine